Assays

These assays are methods developed for quantifying the amount and activity of DHPS.

• Dramatic attenuation of hypusine formation on eukaryotic initiation factor 5A during senescence of IMR-90 human diploid fibroblasts. (PMID: 9066781,(ncbi.nlm.nih.gov)) Chen Z.P. … Chen K.Y. J. Cell. Physiol. 1997 22 64
  • An in vitro cross-labeling assay was developed for simultaneous measurement of the DHPS activity and protein substrate (elF-5A precursor) amount. Researchers used this method to show that the age-dependent attenuation of elF-5A precursor protein may be regulated at either translational or post-translational level.
• Evaluation of the metal ion requirement of the human deoxyhypusine hydroxylase from HeLa cells using a novel enzyme assay. (PMID: 8601426,(ncbi.nlm.nih.gov)) Csonga R. … Klier H. FEBS Lett. 199622 64
  • A quick method for monitoring the deoxyhypusine hydroxylase activity was established, employing the oxidative cleavage of the hypusyl residue and extraction of the generated aldehydes. A ferric ion was determined to be essential for enzymatic activity.
• Deoxyhypusine synthase assay based on the use of polyhistidine-tagged substrate and metal chelate-affinity chromatography. (PMID: 7985780,(ncbi.nlm.nih.gov)) Tao Y. … Chen K.Y. Anal. Biochem. 199422 64
  • A rapid and sensitive assay for deoxyhypusine synthase was developed utilizing a microscale metal-affinity chromatography.